LrpC

• Description: sequence-independent DNA-binding and DNA-bending protein, facilitates the formation of higher order protein-DNA complexes
  
  

Gene name	lrpC
Synonyms	ydaI
Essential	no
Product	DNA-binding and -bending protein
Function	DNA repair and recombination
MW, pI	16 kDa, 7.743
Gene length, protein length	432 bp, 144 aa
Immediate neighbours	ydzA, topB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2CFX

• UniProt: P96582

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lrpC PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: 50 ... 300 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paul Thaw, Svetlana E Sedelnikova, Tatyana Muranova, Sebastian Wiese, Sylvia Ayora, Juan C Alonso, Arie B Brinkman, Jasper Akerboom, John van der Oost, John B Rafferty
Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family.
Nucleic Acids Res: 2006, 34(5);1439-49
[PubMed:16528101] [WorldCat.org] [DOI] (I e)

Gema López-Torrejón, María I Martínez-Jiménez, Silvia Ayora
Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination.
Nucleic Acids Res: 2006, 34(1);120-9
[PubMed:16407330] [WorldCat.org] [DOI] (I e)

Christophe Beloin, Josette Jeusset, Bernard Revet, Gilles Mirambeau, Francoise Le Hégarat, Eric Le Cam
Contribution of DNA conformation and topology in right-handed DNA wrapping by the Bacillus subtilis LrpC protein.
J Biol Chem: 2003, 278(7);5333-42
[PubMed:12458218] [WorldCat.org] [DOI] (P p)

C Beloin, R Exley, A L Mahé, M Zouine, S Cubasch, F Le Hégarat
Characterization of LrpC DNA-binding properties and regulation of Bacillus subtilis lrpC gene expression.
J Bacteriol: 2000, 182(16);4414-24
[PubMed:10913073] [WorldCat.org] [DOI] (P p)

A Tapias, G López, S Ayora
Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein which bridges DNA.
Nucleic Acids Res: 2000, 28(2);552-9
[PubMed:10606655] [WorldCat.org] [DOI] (I p)

C Beloin, S Ayora, R Exley, L Hirschbein, N Ogasawara, Y Kasahara, J C Alonso, F L Hégarat
Characterization of an lrp-like (lrpC) gene from Bacillus subtilis.
Mol Gen Genet: 1997, 256(1);63-71
[PubMed:9341680] [WorldCat.org] [DOI] (P p)