HutI

• Description: imidazolone-5-propionate hydrolase
  
  

Gene name	hutI
Synonyms
Essential	no
Product	imidazolone-5-propionate hydrolase
Function	histidine utilization
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	45 kDa, 5.215
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	hutU, hutG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H₂O = N-formimidoyl-L-glutamate + H⁺ (according to Swiss-Prot)

• Protein family: hutI family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2BB0

• UniProt: P42084

• KEGG entry: [3]

• E.C. number: 3.5.2.7

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by histidine (HutP) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed
  • HutP: transcriptional antitermination at a protein-dependent RNA switch located between hutP and hutH PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: