NrgA

• Description: ammonium transporter, required at low ammonium concentration
  
  

Gene name	nrgA
Synonyms	amtB
Essential	no
Product	ammonium transporter
Function	ammonium uptake
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	42 kDa, 5.727
Gene length, protein length	1212 bp, 404 aa
Immediate neighbours	ywoB, nrgB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36510

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• Structure: 2NUU (the complex with NrgB, from E. coli) PubMed

• UniProt: Q07429

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB

• Sigma factor: SigA

• Regulation: induced by ammonium limitation
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism: transcription activation by TnrA
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP168 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Original Publications

Matthew J Conroy, Anne Durand, Domenico Lupo, Xiao-Dan Li, Per A Bullough, Fritz K Winkler, Mike Merrick
The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.
Proc Natl Acad Sci U S A: 2007, 104(4);1213-8
[PubMed:17220269] [WorldCat.org] [DOI] (P p)

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076] [WorldCat.org] [DOI] (P p)

Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685] [WorldCat.org] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935] [WorldCat.org] [DOI] (P p)