Difference between revisions of "CapC"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[capB]]-[[capC]]-[[capA]]-[[ywtC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed]
+
* '''Operon:''' ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed]
  
 
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19420703 PubMed]
 
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19420703 PubMed]

Revision as of 10:57, 9 January 2010

  • Description: capsular polyglutamate biosynthesis

Gene name capC
Synonyms ywtA, pgsC
Essential no
Product unknown
Function unknown
MW, pI 16 kDa, 9.672
Gene length, protein length 447 bp, 149 aa
Immediate neighbours capA, capB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwtA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU35890

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703] [WorldCat.org] [DOI] (I p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Characterization of the Bacillus subtilis ywsC gene, involved in gamma-polyglutamic acid production.
J Bacteriol: 2002, 184(2);337-43
[PubMed:11751809] [WorldCat.org] [DOI] (P p)