Difference between revisions of "DegQ"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' [[degQ]] {{PubMed|3007431}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3007431}} |
| − | * '''Regulation:''' | + | * '''Regulation:''' |
| + | ** induced by high cell density ([[ComA]]) {{PubMed|1901055}} | ||
| + | ** subject to carbon catabolite repression {{PubMed|1901055}} | ||
| + | ** controlled by [[DegU]] {{PubMed|1901055}} | ||
| − | * '''Regulatory mechanism:''' [[ComA]]: transcription activation | + | * '''Regulatory mechanism:''' |
| + | ** [[ComA]]: transcription activation {{PubMed|1901055}} | ||
| + | ** [[DegU]]: transcription activation {{PubMed|1901055}} | ||
* '''Additional information:''' the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed] | * '''Additional information:''' the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed] | ||
| Line 120: | Line 125: | ||
=References= | =References= | ||
| − | <pubmed>17850253,2428811,, 19447339 </pubmed> | + | <pubmed>17850253,2428811,, 19447339 3007431 1901055 16091050 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:28, 28 December 2009
- Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
| Gene name | degQ |
| Synonyms | sacQ |
| Essential | no |
| Product | pleiotropic regulator |
| Function | regulation of exoenzyme synthesis |
| MW, pI | 5 kDa, 6.536 |
| Gene length, protein length | 138 bp, 46 aa |
| Immediate neighbours | comQ, yuzC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU31720
Phenotypes of a mutant
loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: degQ family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: Q99039
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
Biological materials
- Mutant: QB4851 (cat), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nobuchika Takesue, Teruo Sone, Michiko Tanaka, Fusao Tomita, Kozo Asano
Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system.
J Biosci Bioeng: 2009, 107(6);623-9
[PubMed:19447339]
[WorldCat.org]
[DOI]
(I p)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
Nicola R Stanley, Beth A Lazazzera
Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly-gamma-dl-glutamic acid production and biofilm formation.
Mol Microbiol: 2005, 57(4);1143-58
[PubMed:16091050]
[WorldCat.org]
[DOI]
(P p)
T Msadek, F Kunst, A Klier, G Rapoport
DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ.
J Bacteriol: 1991, 173(7);2366-77
[PubMed:1901055]
[WorldCat.org]
[DOI]
(P p)
H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811]
[WorldCat.org]
[DOI]
(P p)
M Yang, E Ferrari, E Chen, D J Henner
Identification of the pleiotropic sacQ gene of Bacillus subtilis.
J Bacteriol: 1986, 166(1);113-9
[PubMed:3007431]
[WorldCat.org]
[DOI]
(P p)
