PckA

• Description: phosphoenolpyruvate carboxykinase
  
  

Gene name	pckA
Synonyms	ppc
Essential	no
Product	phosphoenolpyruvate carboxykinase
Function	synthesis of phosphoenolpyruvate
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	58,1 kDa, 5.12
Gene length, protein length	1581 bp, 527 amino acids
Immediate neighbours	metK, ytmB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO₂ (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)

• Protein family: phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • Nucleotide binding Domain (233–240)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

• Structure: 2PXZ (E.coli)

• Swiss prot entry: P54418

• KEGG entry: [3]

• E.C. number: 4.1.1.49

Expression and regulation

• Operon:

• Sigma factor: SigA PubMed

• Regulation: repressed by glucose (7-fold) PubMed, repressed (35-times) Glc, repressor CcpN PubMed

• Regulatory mechanism: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France