AbbA

• Description: inhibitor of AbrB activity
  
  

Gene name	abbA
Synonyms	ykzF
Essential	no
Product	anti-repressor
Function	inhibition of AbrB
Gene expression levels in SubtiExpress: abbA
Interactions involving this protein in SubtInteract: AbbA
MW, pI	7 kDa, 4.642
Gene length, protein length	195 bp, 65 aa
Immediate neighbours	ykuK, ykuL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control

This gene is a member of the following regulons

CcpA regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU14120

Database entries

• BsubCyc: BSU14120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibition of AbrB activity PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • AbbA-AbrB, AbbA binds the N-terminal DNA-binding domain of AbrB PubMed

• Localization:

Database entries

• BsubCyc: BSU14120

• Structure: 2LZF PubMed

• UniProt: O31697

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ykuJ-ykuK-abbA-ykuL-ccpC PubMed

• Expression browser: abbA PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (4.3-fold) (CcpA) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Spo0A: transcription activation by Spo0A~P PubMed

• Additional information:

Biological materials

• Mutant: GP214 (deletion of ykuJ-ykuK-abbA-ykuL, replaced by aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP708 (in pAC5), available in Stülke lab, pGP712 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage