panB
168
alpha-ketoisovalerate hydroxymethyltransferase
locus
BSU_22430
Molecular weight
29.61 kDa
pI
5.28
function
biosynthesis of coenzyme A
product
3-methyl-2-oxobutanoate hydroxymethyltransferase
essential
quasi
ec
2.1.2.11
synonyms
panB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0413 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,353,839 2,354,672
Phenotypes of a mutant
quasi-essential, the mutant is auxotrophic for pantothenate [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
the mutant is viable if it acquires mutations ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR] or [gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]) that allow constitutive expression of [gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN] for the uptake of cysteinopantetheine [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
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The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate (according to UniProt)
Protein family
PanB family (single member, according to UniProt)
Structure
[PDB|3EZ4] (from ''Burkholderia pseudomallei'', 44% identity, 64% similarity)
[AF|P52996]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
Biological materials
Mutant
GP4401 (Δ[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::''neo''), available in [wiki|Jörg Stülke]'s lab
PA126 (''panB''::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE22430 ([gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG, downstream forward: _UP4_GTGCTTGACGGCTTGTACGG
BKK22430 ([gene|A0001681244113B808757281C1B70C0C94F28A27|panB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCTCCTCCTCATG, downstream forward: _UP4_GTGCTTGACGGCTTGTACGG
References
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Time of last update: 2024-09-22 17:47:34
Author of last update: Jstuelk