rok
168
chromosomal loop anchor, repressor of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], nucleoid associated protein that serves to help repress expression of A T-rich genes, many of which appear to have been acquired by horizontal gene transfer
locus
BSU_14240
Molecular weight
21.69 kDa
pI
9.7
function
control of genome organization, silencing of horizontally acquired genes
product
chromosomal loop anchor
essential
no
synonyms
rok, ykuW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
1,493,787 1,494,362
Phenotypes of a mutant
higher excision rate of the conjugative transposon ICEBs1 [Pubmed|21085634]
Advertisement
The protein
Catalyzed reaction/ biological activity
binds to extended regions of the ''B. subtilis'' genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer [Pubmed|21085634]
[wiki|Domains]
N-terminal multimerization domain [pubmed|35075232]
C-terminal DNA-binding domain [pubmed|35075232]
Structure
[PDB|5ZUX] (complexed with DNA) [Pubmed|30252102]
[PDB|5ZUZ] [Pubmed|30252102]
[AF|O34857]
Effectors of protein activity
activity is modulated by interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|27902860]
Expression and Regulation
Operons
genes
[gene|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]
description
[Pubmed|12354229]
regulation
repressed by [protein|search|Rok] [Pubmed|11849533]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|11849533], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|11849533], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|11849533], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Biological materials
Mutant
MGNA-B345 (ykuW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1344 NBRP B. subtilis, Japan]
BKE14240 ([gene|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTCAATGTACCCCCT, downstream forward: _UP4_TAAATATAAAGAAAAACTGC
BKK14240 ([gene|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTCAATGTACCCCCT, downstream forward: _UP4_TAAATATAAAGAAAAACTGC
References
Reviews
Original Publications
Page visits: 4263
Time of last update: 2024-07-13 10:28:18
Author of last update: Jstuelk