ysxC

ysxC
168

assembly of the 50S subunit of the ribosome

Locus
BSU_28190
Molecular weight
21.88 kDa
Isoelectric point
9.71
Protein length
195 aaSequenceBlast
Gene length
588 bpSequenceBlast
Function
ribosome assembly
Product
GTPase
Essential
yes
Synonyms
ysxC, orfX, engB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0218 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,879,882 → 2,880,469
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes GTP and readily exchanges GDP for GTP
participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit PubMed
Protein family
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily (according to UniProt)
Domains
EngB-type G (aa 22-195) (according to UniProt)
Structure
1SVI (PDB) (complex with GDP) PubMed
1SUL (PDB) PubMed
P38424 (AlphaFold)
Paralogous protein(s)
RbgA, ThdF, Obg, YqeH, YnbA, EngA, YyaF, Era
Localization
cytosol (according to UniProt)
Expression and Regulation
Operons
Genes
lonA-ysxC
Description
PubMed
Regulation
induced by heat stress (CtsR) PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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lonA→ysxC

2025-03-25 19:10:29

ghost

105

4cb14aa2acaf53ec467aec28bb01e2f09b1393ff

7EEAAFADAFDA76CE2520B524F32B86123E1933A6

Biological materials
Mutant
MGNA-A996 (ysxC::erm), available at the NBRP B. subtilis, Japan
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
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Original Publications
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DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C

Page visits: 5470

Time of last update: 2025-04-01 20:15:57

Author of last update: Jstuelk