lipM

lipM
168

octanoyltransferase

Locus
BSU_24530
Molecular weight
32.09 kDa
Isoelectric point
5.93
Protein length
278 aaSequenceBlast
Gene length
837 bpSequenceBlast
Function
biosynthesis of lipoic acid
Product
octanoyltransferase
Essential
no
E.C.
2.3.1.181
Synonyms
lipM, yqhM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0095 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,543,968  2,544,804
The protein
Catalyzed reaction/ biological activity
transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-LipM intermediate PubMed
L-lysyl-[protein] + octanoyl-[ACP] --> H+ + holo-[ACP] + N6-octanoyl-L-lysyl-[protein] (according to UniProt)
Protein family
octanoyltransferase LipM family (single member, according to UniProt)
Domains
BPL/LPL catalytic domain (aa 33-248) (according to UniProt)
Structure
3A7A (PDB) (from E. coli, 25% identity) PubMed
P54511 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
lipM-mntR
Description
PubMed
Open in new tab

lipM->mntR

2025-03-31 23:42:39

Jstuelk

72

43142196e46b9630ee956a30cb1c43887eb0465a

68845A224A7971C2B25ADC30013D1BE3E2F7504C

Biological materials
Mutant
MGNA-C469 (yqhM::erm), available at the NBRP B. subtilis, Japan
BKE24530 (lipM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC,  downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
BKK24530 (lipM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC,  downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
References
Reviews
Loading
Original Publications
Loading

19CEB80CF637F1FED7C493EF743B4BFA632C8D44

Page visits: 3162

Time of last update: 2025-04-10 05:47:56

Author of last update: Jstuelk