nrnB

nrnB
168

sporulation-specific 3′-5′ exonuclease, degradation of pGpG

Locus
BSU_18200
Molecular weight
46.28 kDa
Isoelectric point
5.03
Protein length
399 aaSequenceBlast
Gene length
1200 bpSequenceBlast
Function
degradation of RNA oligonucleotides
Product
sporulation-specific 3′-5′ exonuclease
Essential
no
Synonyms
nrnB, yngD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2404 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,948,313 → 1,949,512
The protein
Catalyzed reaction/ biological activity
degradation of RNA (both oligonucleotides and longer RNAs) (3′-5′ exonuclease activity) PubMed
degradation of pGpG derived from c-di-GMP PubMed
Protein family
DHH/DHHA1 protein family of phosphoesterases PubMed
Domains
DHH-DHHA1 domain PubMed
Structure
O31824 (AlphaFold)
Expression and Regulation
Operons
Genes
nrnB
Description
PubMed
Regulation
expressed during sporulation in the forespore PubMed
Regulatory mechanism
SpoVT: repression, PubMed, in spoVT regulon
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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nrnB

2025-04-08 21:36:43

Jstuelk

99

9D16D4BCECFCB8D5E0C6FC3E4116A1AECC6E975D

11888C9635991429F9F7509AF886B91086EAA182

Biological materials
Mutant
MGNA-B398 (yngD::erm), available at the NBRP B. subtilis, Japan
BKE18200 (ΔnrnB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT,  downstream forward: _UP4_TAAAAAAATATGCATAGGGG
BKK18200 (ΔnrnB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT,  downstream forward: _UP4_TAAAAAAATATGCATAGGGG
References
Reviews
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Original Publications
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BBB0848B2516188E950763E1D0194860923CE895

Page visits: 2963

Time of last update: 2025-04-08 09:58:46

Author of last update: Jstuelk