pgm

pgm
168

phosphoglycerate mutase, glycolytic / gluconeogenic enzyme

Locus
BSU_33910
Molecular weight
56.14 kDa
Isoelectric point
5.21
Protein length
511 aaSequenceBlast
Gene length
1536 bpSequenceBlast
Function
enzyme in glycolysis / gluconeogenesis
Product
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Essential
Yes
E.C.
5.4.2.1
Synonyms
pgm, gpmI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0696 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CggR regulon

Gene
Coordinates
3,477,877 3,479,412
Phenotypes of a mutant
Essential PubMed
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> 3-phosphoglycerate (according to UniProt)
Protein family
BPG-independent phosphoglycerate mutase family (single member, according to UniProt)
Cofactors
Mn2+
Structure
1EJJ (PDB) (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), 1EQJ (PDB) (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), Geobacillus stearothermophilus, complex with 2-phosphoglycerate NCBI, Geobacillus stearothermophilus, complex with 3-phosphoglycerate NCBI
P39773 (AlphaFold)
Modification
phosphorylation on Ser-62 PubMed, PubMed
Effectors of protein activity
Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione PubMed
2,3-Diphosphoglycerate has NO role on this enzyme regulation PubMed
Kinetic information
Reversible Michaelis-Menten PubMed
Localization
Cytoplasm (Homogeneous) PubMed
Additional information
extensive information on the structure and enzymatic properties of Pgm can be found at Proteopedia
Expression and Regulation
Operons
Genes
cggR-gapA-pgk-tpi-pgm-eno
Description
PubMed
Regulation
expression induced by glycolytic intermediates (CggR) CggR PubMed
the mRNA is processed between cggR and gapA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
CggR: repression, PubMed, in cggR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

cggR→eno

2025-03-27 06:06:35

Jstuelk

174

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

Genes
pgk-tpi-pgm-eno
Description
PubMed
Open in new tab

pgk→eno

2025-04-03 13:01:53

Jstuelk

130

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP593 (pgm::cat), available in Jrg Stlke's lab, PubMed
GP598 (pgm::erm), available in Jrg Stlke's lab, PubMed
GP698 (pgm-eno::cat), available in Jrg Stlke's lab, PubMed
Expression vectors
pGP1425 (expression of pgm in B. subtilis, in pBQ200), available in Jrg Stlke's lab
pGP1500 (expression of pgm and eno in B. subtilis, in pBQ200), available in Jrg Stlke's lab
pGP1101 (N-terminal His-tag, in pWH844), available in Jrg Stlke's lab
pGP396 (Pgm-S62A, N-terminal His-tag, in pWH844), available in Jrg Stlke's lab
pGP92 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jrg Stlke's lab, PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab, PubMed
Labs working on this gene/protein
Jrg Stlke, University of Gttingen, Germany Homepage
Mark J. Jedrzejas, Research Center Oakland, CA, USA Homepage
References
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Time of last update: 2025-04-01 20:01:49

Author of last update: Jstuelk