ald

ald
168

L-alanine dehydrogenase

Locus
BSU_31930
Molecular weight
39.53 kDa
Isoelectric point
5.12
Protein length
Gene length
Function
alanine utilization
Product
L-alanine dehydrogenase
Essential
no
E.C.
1.4.1.1
Synonyms
ald, spoVN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0686 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,278,325  3,279,461
Phenotypes of a mutant
premature spore germination due to alanine accumulation, activation of germination via GerAA-GerAB-GerAC PubMed
The protein
Catalyzed reaction/ biological activity
H2O + L-alanine + NAD+ --> H+ + NADH + NH4+ + pyruvate (according to UniProt)
Protein family
AlaDH/PNT family (single member, according to UniProt)
NADH PubMed
Structure
2EEZ (PDB) (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
Description
Open in new tab

ald-yukJ

2025-01-14 05:28:40

Jstuelk

96

a0342f1f205dcf32dcba8c3ce824f96bdadfef08

3D4016F1849C95234C58E63816F159C1368DFC90

Genes
Description
Regulation
induced by alanine (AdeR) PubMed
Regulatory mechanism
AdeR: activation, PubMed, in adeR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

ald

2025-01-17 08:55:18

ghost

93

8e5040b90dc0307ee274496e90c947f6e031824f

41BB162676969B273D29E5ECC73B26F6E7487C62

Additional information
expression is reduced in motile cells as compared to non-motile cells PubMed
Biological materials
Mutant
GP3596 (Δald::tet), available in Jörg Stülke's lab
BKE31930 (ald::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA,  downstream forward: _UP4_TAATTCACAATAAGCTTGCA
BKK31930 (ald::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA,  downstream forward: _UP4_TAATTCACAATAAGCTTGCA
Expression vectors
pGP4001: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
Two-hybrid system
FLAG-tag construct
GP4486 ald-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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2D346CF412BA32EE18111DA930CFA1306F95024B

Page visits: 4887

Time of last update: 2025-01-21 10:04:32

Author of last update: Robert.warneke