ald
168
L-alanine dehydrogenase
Locus
BSU_31930
Molecular weight
39.53 kDa
Isoelectric point
5.12
Function
alanine utilization
Product
L-alanine dehydrogenase
Essential
no
E.C.
1.4.1.1
Synonyms
ald, spoVN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0686 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,278,325 3,279,461
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
H2O + L-alanine + NAD+ --> H+ + NADH + NH4+ + pyruvate (according to UniProt)
Protein family
AlaDH/PNT family (single member, according to UniProt)
NADH PubMed
Structure
2EEZ (PDB) (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Additional information
expression is reduced in motile cells as compared to non-motile cells PubMed
Biological materials
Mutant
GP3596 (Δald::tet), available in Jörg Stülke's lab
Expression vectors
pGP4001: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
Two-hybrid system
FLAG-tag construct
References
Reviews
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Original Publications
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Time of last update: 2025-01-21 10:04:32
Author of last update: Robert.warneke