spoIIIM

spoIIIM
168

sporulation protein, required for the stabilization of septal pore during engulfment

Locus
BSU_25060
Molecular weight
10.63 kDa
Isoelectric point
8.09
Protein length
99 aaSequenceBlast
Gene length
300 bpSequenceBlast
Function
closure of septal pore
Product
single LysM domain-containing protein
Essential
no
E.C.
null
Synonyms
yqfZ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
2,588,701  2,589,000
Phenotypes of a mutant
a pbpG spoIIIM double mutant has a reduced sporulation efficiency (8.8%) PubMed
a pbpG spoIIIM double mutant exhibits a severe miscompartmentalization defect PubMed
a pbpG spoIIIM double mutant exhibits severe spore morphological defects PubMed
The protein
Catalyzed reaction/ biological activity
stabilization of septal pore during engulfment PubMed
Domains
N-terminal transmembrane segment PubMed
contains a N-acetylglucosamine-polymer-binding LysM domain (aa 48-95) PubMed
Structure
P54483 (AlphaFold)
Localization
forms a septal focus in the engulfing membrane, this depends on SpoIIIE
Expression and Regulation
Operons
Genes
spoIIIM-ispG
Description
PubMed
Regulation
expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed
Regulatory mechanism
SpoIIID: repression, PubMed, in spoIIID regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
Open in new tab

spoIIIM→ispG

2025-03-16 18:29:38

ghost

83

7f29277c4da18ecbbf9247ac151795205592bbce

5FAA9F6ECFC53D5F888A9A3A86FCBB60ECB3EA36

Biological materials
Mutant
MGNA-C488 (yqfZ::erm), available at the NBRP B. subtilis, Japan
BKE25060 (spoIIIM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGCTTTTCCTCCCGCAT,  downstream forward: _UP4_TAATTTAACGTTAATTTCTT
BKK25060 (spoIIIM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGCTTTTCCTCCCGCAT,  downstream forward: _UP4_TAATTTAACGTTAATTTCTT
References
Reviews
Loading
Original Publications
Loading

144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885

Page visits: 2392

Time of last update: 2025-04-11 07:14:32

Author of last update: Jstuelk