gcvT

gcvT
168

aminomethyltransferase (glycine cleavage system protein T)

Locus
BSU_24570
Molecular weight
39.62 kDa
Isoelectric point
5.73
Protein length
362 aaSequenceBlast
Gene length
1089 bpSequenceBlast
Function
glycine utilization
Product
aminomethyltransferase (glycine cleavage system protein T)
Essential
no
E.C.
2.1.2.10
Synonyms
gcvT, yqhI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0404 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,548,245 → 2,549,333
The protein
Catalyzed reaction/ biological activity
(6S)-5,6,7,8-tetrahydrofolate + (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[protein] --> (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N6-dihydrolipoyl-L-lysyl-[protein] + NH4+ (according to UniProt)
Protein family
gcvT family (single member, according to UniProt)
Cofactors
tetrahydrofolate
Structure
1YX2 (PDB)
P54378 (AlphaFold)
Expression and Regulation
Operons
Genes
gcvT-gcvPA-gcvPB
Description
PubMed
Regulation
induced by glycine PubMed
the Gly-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
Gly-box: RNA switch, PubMed, in Gly-box
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gcvT→gcvPB

2025-03-07 03:02:42

Jstuelk

77

b8399a8069749ebce96a5673beef55862123ad69

18A61526FCBD278A97D718FA1F476B3A75682C1F

Biological materials
Mutant
MGNA-C428 (yqhI::erm), available at the NBRP B. subtilis, Japan
BKE24570 (ΔgcvT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA,  downstream forward: _UP4_TAATATTTTTTCTTGGAGAG
BKK24570 (ΔgcvT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAATTCCTCCCCTTTA,  downstream forward: _UP4_TAATATTTTTTCTTGGAGAG
LacZ fusion
pGP431 (in pAC7), available in Stülke lab
References
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F6F449170276055E6D60D89E9E17A152A82CA166

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Time of last update: 2025-04-05 19:18:29

Author of last update: Jstuelk