yjbO

yjbO
168

pseudouridylate synthase, pseudouridylation of position 31 (and also 32) in several tRNAs

Locus
BSU_11620
Molecular weight
31.32 kDa
Isoelectric point
6.55
Protein length
283 aaSequenceBlast
Gene length
852 bpSequenceBlast
Function
RNA modification
Product
pseudouridylate synthase
Essential
no
Synonyms
yjbO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,238,523  1,239,374
The protein
Catalyzed reaction/ biological activity
uridine in RNA --> ψ-uridine at position 31 in several tRNAs PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with YhcT and RluD, according to UniProt)
Domains
S4 RNA-binding domain (aa 25-79) (according to UniProt)
Structure
1V9F (PDB) (RluD from E. coli, 40% identical, aa 64 - 279) PubMed
O31613 (AlphaFold)
Paralogous protein(s)
RluD, YhcT
Expression and Regulation
Operons
Genes
yjbL-sasB-nadF-yjbO
Description
PubMed
Regulation
expressed during exponential growth
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yjbL→yjbO

2025-04-03 22:19:01

Jstuelk

101

c5edd61e714281007d4d9ec99a1efa0cfef63f09

83E46551CBC32AB07130281546FE3D422612C2C3

Biological materials
Mutant
MGNA-B161 (yjbO::erm), available at the NBRP B. subtilis, Japan
BKE11620 (yjbO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
BKK11620 (yjbO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
References
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2AECDA9C4D0E704976B9E2168722EC80FE7A256D

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Time of last update: 2025-04-02 18:00:43

Author of last update: Jstuelk