hemAT

hemAT
168

soluble chemotaxis receptor, heme-containing O2 sensor protein, senses ethanol and other short-chain alcohols

Locus
BSU_10380
Molecular weight
48.60 kDa
Isoelectric point
5.44
Protein length
432 aaSequenceBlast
Gene length
1299 bpSequenceBlast
Function
movement towards oxygen
Product
haem-based aerotactic transducer
Essential
no
Synonyms
hemAT, yhfV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,112,620 → 1,113,918
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
NCIB3610 hemAT mutant has higher fitness than ''hag'' mutant during pellicle formation PubMed
NCIB3610 hemAT-''hag double mutant has similar fitness to single hag'' mutant during pellicle formation PubMed
Additional information
the gene readily acquires mutations during adaptation to growth as pellicle biofilm PubMed
The protein
Catalyzed reaction/ biological activity
required for full expression of the ''phoD-tatAD-tatCD'' operon PubMed
responsible for attractant response towards phenol and other aromatics at low (micromolar) concentrations PubMed
Domains
Methyl-accepting transducer domain (aa 184-420) (according to UniProt)
Cofactors
heme
Structure
1OR4 (PDB) (in cyano-liganded form),  1OR6 (PDB)\tPubMed
O07621 (AlphaFold)
Localization
forms clusters at the cell poles PubMed
homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase PubMed
polar foci in individual cells at later growth stages PubMed
Expression and Regulation
Operons
Genes
hemAT
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Additional information
in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
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hemAT

2025-03-18 21:52:42

ghost

92

f64f99af2dc569ae285b12dce23ed8c91b8d1973

0E251385AD9FC5660DBAE2D8740FCC57A77EA586

Biological materials
Mutant
MGNA-B282 (yhfV::erm), available at the NBRP B. subtilis, Japan
TB239 (hemAT::neo in 168) PubMed
TB241 (hemAT::neo in NCIB3610) PubMed
BKE10380 (ΔhemAT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATACAATGATCCCCCTTG,  downstream forward: _UP4_TAACCATCAAAAACCGGTCT
BKK10380 (ΔhemAT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATACAATGATCCCCCTTG,  downstream forward: _UP4_TAACCATCAAAAACCGGTCT
Labs working on this gene/protein
Akos T Kovacs
References
Reviews
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Original Publications
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Time of last update: 2025-04-04 00:29:03

Author of last update: Jstuelk