alkA

alkA
168

DNA-3-methyladenine glycosylase

Locus
BSU_01800
Molecular weight
35.48 kDa
Isoelectric point
8.15
Protein length
303 aaSequenceBlast
Gene length
912 bpSequenceBlast
Function
adaptive response to alkylative DNA damage
Product
DNA-3-methyladenine glycosylase
Essential
no
E.C.
3.2.2.21
Synonyms
alkA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
202,547  203,458
The protein
Catalyzed reaction/ biological activity
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)
Protein family
alkylbase DNA glycosidase AlkA family (with YfjP, according to UniProt)
Structure
3OH6 (PDB) (from E. coli, 25.1% identity) PubMed
P37878 (AlphaFold)
Expression and Regulation
Operons
Genes
alkA
Description
PubMed
Regulatory mechanism
AdaA: activation, in adaA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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alkA

2025-03-17 14:51:22

ghost

85

676a0907f9180d6e66d70f7c75e6e39c4b25e7f0

53C1A72D1C318FDC32AFE83167CE6185E9FCC428

Biological materials
Mutant
BKE01800 (alkA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT,  downstream forward: _UP4_TGAATCATTTCAACTTTAAC
BKK01800 (alkA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT,  downstream forward: _UP4_TGAATCATTTCAACTTTAAC
References
Reviews
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Original Publications
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297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509

Page visits: 3074

Time of last update: 2025-04-03 12:53:26

Author of last update: Jstuelk