ispF

ispF
168

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

Locus
BSU_00910
Molecular weight
16.98 kDa
Isoelectric point
5.4
Protein length
158 aaSequenceBlast
Gene length
477 bpSequenceBlast
Function
MEP pathway of isoprenoid biosynthesis
Product
2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
Essential
yes
E.C.
4.6.1.12
Synonyms
ispF, yacN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0245 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
110,480  110,956
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
4-CDP-2-C-methyl-D-erythritol 2-phosphate --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP (according to UniProt)
Protein family
IspF family (single member, according to UniProt)
Structure
5IWX (PDB) (native) PubMed
5IWY (PDB) (complex with CMP) PubMed
Q06756 (AlphaFold)
Expression and Regulation
Operons
Genes
yacL-ispD-ispF
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
SigM: sigma factor, PubMed, in sigM regulon
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yacL→ispF

2025-04-04 00:34:02

ghost

91

7dc686870ed58f71ca9e5b0841c69a922f74d84a

EFDC5F8C8F63B342C29A9AB39CD8005147D16917

Biological materials
Mutant
MGNA-B882 (yacN::erm), available at the NBRP B. subtilis, Japan
References
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1A6793A3A5AC568C67873C8708E9F581F42AB9BA

Page visits: 6287

Time of last update: 2025-04-04 00:39:35

Author of last update: Melvin.boenninger