TagA

• Description: UDP-N-acetyl-D-mannosamine transferase
  
  

Gene name	tagA
Synonyms
Essential	yes PubMed, no PubMed
Product	UDP-N-acetyl-D-mannosamine transferase
Function	biosynthesis of teichoic acid
MW, pI	29 kDa, 10.133
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	tagD, tagB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35750

Phenotypes of a mutant

essential PubMed, reduced growth rate, loss of rod-like morphology PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetyl-D-mannosamine + N-acetyl-D-glucosaminyldiphosphoundecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol (according to Swiss-Prot)

• Protein family: glycosyltransferase 26 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P27620

• KEGG entry: [3]

• E.C. number: 2.4.1.187

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michael A D'Elia, James A Henderson, Terry J Beveridge, David E Heinrichs, Eric D Brown
The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus.
J Bacteriol: 2009, 191(12);4030-4
[PubMed:19376878] [WorldCat.org] [DOI] (I p)