Mbl

• Description: cell-shape determining protein
  
  

Gene name	mbl
Synonyms
Essential	no
Product	MreB-like protein
Function	cell-shape determination
MW, pI	35 kDa, 5.669
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	flhO, spoIIID
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

non-viable in the presence of low Mg(2+), readily accumulate rsgI suppressor mutants PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): MreB, MreBH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MreB-Mbl, Mbl-MreBH PubMed

• Localization: forms helical structures close to the inner surface of the cytoplasmic membrane PubMed

Database entries

• Structure:

• Swiss prot entry: P39751

• KEGG entry: BSU36410

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in the Jeff Errington and Peter Graumann labs

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

1. Carballido-Lopez, R., Formstone, A., Li, Y., Ehrlich, S. D., and Errington, J. (2006) Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE. Dev. Cell 11: 399-409. PubMed
2. Defeu Soufo, H. J., and P. Graumann. 2006. Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the funtion of MreB. Mol. Microbiol. 62: 1340-1356. PubMed
3. Schirner K, Errington J. (2009) Cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in Bacillus subtilis. J. Bacteriol. 191: 1404-1413. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed