PurR

• Description: transcription repressor of the pur operon
  
  

Gene name	purR
Synonyms	yabI
Essential	no
Product	transcription repressor of the pur operon
Function	regulation of purine biosynthesis
MW, pI	31 kDa, 5.771
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	ispE, yabJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Biological activity: transcriptional repressor of purine biosynthetic operons

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

• Interactions: PurR-Prs

• Localization:

Database entries

• Structure: 1P4A (complex with PRPP) NCBI PubMed

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

• Operon: purR-yabJ PubMed

• Sigma factor:

• Regulation: negative autoregulation PubMed

• Regulatory mechanism: repression by PurR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Weng et al. (1995) Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92: 7455-7459. PubMed
2. Weng & Zalkin (2000) Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.Currr. Microbiol. 41: 56-59. PubMed
3. Sinha et al. (2003) The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.J. Bacteriol. 185: 4087-4098. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed