PWH844

pWH844: click to enlarge

pWH844 cloning region: click to enlarge

vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Hillen lab

in E. coli: ampicillin resistance

similar plasmid: pGP570

sequencing primer:

• JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
• SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
• PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
• pWH844 fw: 5’-TATGAGAGGATCGCATCACCAT-3‘

Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed