List of plasmids

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

pAC5: translational lacZ-fusions (chloramphenicol resistence)
pAC6: transcriptional lacZ-fusions (cat resistence)
pAC7: translational lacZ-fusions (kanamycin resistence)
pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
a suite of vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci PubMed
List of GFP variants useful for various fusions (IPTG inducible Phyperspank with amyE integration) PubMed

pBP43: integrative plasmid for the fusion of monomeric GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, this is the preferred plasmid for constructing GFP fusions!
pGP1871: integrative plasmid for the fusion of YFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter
pGP1080: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, allows express of the downstream gene by the P(spac) promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!
pGP1870: integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promoter, ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!
ectopic integration vectors for generating fluorescent promoter fusions in Bacillus subtilis with minimal dark noise PubMed

pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
pGP1370: replicative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, high expression due to the strong degQ(Hy) promoter from pBQ200

pBP26: expression of YFP in B. subtilis and in E. coli
pBP27: expression of CFP in B. subtilis and in E. coli
pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
pGP886: integration vector (integrates in xkdE); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
pGP888: integration vector (integrates in ganA); allowing the expression of genes under the control of the xylose-inducible PxylA promoter in B. subtilis
pGP1459: integration vector (integrates in ganA) analogous to pGP380; expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
pGP1460: integration vector (integrates in ganA) analogous to pGP382; expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
pGP1389: integrative plasmid for the fusion of a Strep-tag to the C-terminus of a protein, keeping expression under the control of the natural promoter

pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off by thrombin
pETM-11: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off by TEV protease
pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein