PETM-11

pWH844: click to enlarge

• vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease

• host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

• in E. coli: ampicillin resistance

• from EMBL

• sequencing primer:
• CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
• CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘

Annett Dümmler, Ann-Marie Lawrence, Ario de Marco
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.
Microb Cell Fact: 2005, 4;34
[PubMed:16351710] [WorldCat.org] [DOI] (I e)