recA
168

multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage), required to internalize and to recombine ssDNA with homologous resident duplex, required for efficient survival and replication restart after replication-transcription conflicts, crucial for the acquisition of homologous genes from related species by natural transformation

Locus

BSU_16940

Molecular weight

37.93 kDa

Isoelectric point

4.88

Protein length

348 aaSequenceBlast

Gene length

1047 bpSequenceBlast

Function

DNA repair/ recombination

Product

multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)

Essential

Synonyms

recA, recE

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0468 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, ComK regulon, LexA regulon

Gene

Coordinates

1,764,645 1,765,691

Phenotypes of a mutant

slower growth PubMed

drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

reduced sporulation efficiency PubMed

strongly reduced chromosomal transformation rate PubMed

strongly reduced survival after mitomycin treatment PubMed

no amplification of the gltA-gltB chromosomal region to suppress the glutamate auxotrophy of a gltC mutant PubMed

sensitive to Cr(VI) treatment PubMed

reduced resistance towards electron beams PubMed

reduced viability of a rarA recA double mutant PubMed

suppression of lethality of pcrA inactivation PubMed

a recA rnhC double mutant is not viable PubMed

ATP --> ADP + P_i PubMed

The protein

Catalyzed reaction/ biological activity

RecA stimulates ssDNA phosphorylase activity of PnpA PubMed

RecA-ATP in concert with DprA and SsbA catalyzes DNA strand exchange, with SsbB as an accessory factor PubMed

RecA-dATP catalyzes strand exchange even in the absence of the accessory factors PubMed

protects sporulating cells from DNA damage PubMed

contributes to transfection with naked phage DNA PubMed

RecA polymerized on tailed SPP1 duplex intermediates invades a homologous region in another incomplete molecule, and in concert with RecD2 helicase, reconstitutes a complete linear phage genome with redundant regions at the ends of the molecule PubMed

Protein family

RecA family (together with RadA) (according to UniProt)

Structure

1UBC (PDB) (RecA from Mycobacterium smegmatis, 67% identity) PubMed

P16971 (AlphaFold)

Modification

phosphorylated on Arg-58 PubMed

phosphorylated on Ser-2 PubMed by YabT PubMed

Effectors of protein activity

RecO and DprA provide RecA access to ssDNA during chromosomal transformation PubMed

interaction with DisA inhibits RecA filament growth and RecA-mediated DNA strand exchange PubMed

Localization

colocalizes to the replisome in response to endogenous and exogenous DNA damage and in response to damage-independent fork arrest (formation of DNA repair centers), repair center formation depends on RecO and RecR, and is facilitated by RecF and SsbA PubMed

Nucleoid (Mid-cell) PubMed

localizes to one cell pole PubMed

co-localizes with the DNA uptake machinery PubMed

forms a transient, mobile focus associated with the chromosome during spore development PubMed

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

Genes

recA

Description

PubMed