MifM

• Description: ribosome-nascent chain sensor of membrane protein biogenesis
  
  

Gene name	mifM
Synonyms	yqzJ
Essential	no
Product	sensor of SpoIIIJ activity
Function	control of yidC2 translation
Gene expression levels in SubtiExpress: mifM
Interactions involving this protein in SubtInteract: MifM
MW, pI	11 kDa, 5.829
Gene length, protein length	285 bp, 95 aa
Immediate neighbours	polY1, yidC2
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, membrane proteins

The gene

Basic information

• Locus tag: BSU23880

Database entries

• BsubCyc: BSU23880

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal transmembrane domain PubMed
  • the C-terminal domain interacts with the ribosome and arrests the own translation elongation PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel (RplD, RplV) PubMed
  • SpoIIIJ-MifM PubMed
  • RplD-MifM PubMed
  • RplV-MifM PubMed
  • 23S rRNA-MifM PubMed

• Localization:
  • membrane PubMed

Database entries

• BsubCyc: BSU23880

• Structure:

• UniProt: Q7WY64

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: mifM-yidC2 PubMed

• Expression browser: mifM PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mifM PubMed
  • MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
  • elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: