PolY1
- Description: translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci
Gene name | polY1 |
Synonyms | yqjH |
Essential | no |
Product | translesion synthesis (TLS-) DNA polymerase Y1 |
Function | generation of mutations in stationary phase |
Gene expression levels in SubtiExpress: polY1 | |
Interactions involving this protein in SubtInteract: PolY1 | |
Metabolic function and regulation of this protein in SubtiPathways: polY1 | |
MW, pI | 46 kDa, 9.953 |
Gene length, protein length | 1242 bp, 414 aa |
Immediate neighbours | gndA, mifM |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
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Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23870
Phenotypes of a mutant
reduced UV-induced mutagenesis PubMed
Database entries
- BsubCyc: BSU23870
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
- Protein family: DNA polymerase type-Y family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (homogeneous)
Database entries
- BsubCyc: BSU23870
- Structure:
- UniProt: P54545
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Sigma factor:
- Regulation: constitutively expressed PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1111 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications