ScpA

• Description: part of the condensin complex, chromosomal origin condensation and segregation
  
  

Gene name	scpA
Synonyms	ypuG
Essential	yes PubMed
Product	DNA segregation and condensation protein
Function	segregation of replication origins
Gene expression levels in SubtiExpress: scpA
Interactions involving this protein in SubtInteract: ScpA
MW, pI	29 kDa, 4.788
Gene length, protein length	753 bp, 251 aa
Immediate neighbours	scpB, ypuF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU23220

Phenotypes of a mutant

• essential PubMed
• scpA mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

Database entries

• BsubCyc: BSU23220

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: scpA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • part of the condensin complex PubMed
  • present as stable monomer PubMed
  • ScpA-Smc PubMed
  • ScpA-ScpB PubMed

• Localization:
  • nucleoid (Multiple) PubMed
  • the condensin (Smc)2-ScpA-ScpB complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on Spo0J PubMed

Database entries

• BsubCyc: BSU23220

• Structure:
  • 3ZGX (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed
  • 4I98 (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed

• UniProt: P35154

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: scpA-scpB-ypuI PubMed

• Expression browser: scpA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 573 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1071 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Sophie Nolivos, David Sherratt
The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexes.
FEMS Microbiol Rev: 2014, 38(3);380-92
[PubMed:24118085] [WorldCat.org] [DOI] (I p)

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)