Mycoplasma

Methods

Sebastian R Schmidl, Claudine Hames, Jörg Stülke
Expression of Mycoplasma proteins carrying an affinity tag in M. pneumoniae allows rapid purification and circumvents problems related to the aberrant genetic code.
Appl Environ Microbiol: 2007, 73(23);7799-801
[PubMed:17933931] [WorldCat.org] [DOI] (I p)

Sven Halbedel, Jörg Stülke
Tools for the genetic analysis of Mycoplasma.
Int J Med Microbiol: 2007, 297(1);37-44
[PubMed:17223385] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Julia Busse, Sebastian R Schmidl, Jörg Stülke
Regulatory protein phosphorylation in Mycoplasma pneumoniae. A PP2C-type phosphatase serves to dephosphorylate HPr(Ser-P).
J Biol Chem: 2006, 281(36);26253-9
[PubMed:16857667] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Jörg Stülke
Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.
Appl Environ Microbiol: 2006, 72(2);1696-9
[PubMed:16461731] [WorldCat.org] [DOI] (P p)

Claudine Hames, Sven Halbedel, Oliver Schilling, Jörg Stülke
Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.
Appl Environ Microbiol: 2005, 71(7);4097-100
[PubMed:16000825] [WorldCat.org] [DOI] (P p)

The phosphotransferase system

Sven Halbedel, Julia Busse, Sebastian R Schmidl, Jörg Stülke
Regulatory protein phosphorylation in Mycoplasma pneumoniae. A PP2C-type phosphatase serves to dephosphorylate HPr(Ser-P).
J Biol Chem: 2006, 281(36);26253-9
[PubMed:16857667] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Jörg Stülke
Dual phosphorylation of Mycoplasma pneumoniae HPr by Enzyme I and HPr kinase suggests an extended phosphoryl group susceptibility of HPr.
FEMS Microbiol Lett: 2005, 247(2);193-8
[PubMed:15927419] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Claudine Hames, Jörg Stülke
In vivo activity of enzymatic and regulatory components of the phosphoenolpyruvate:sugar phosphotransferase system in Mycoplasma pneumoniae.
J Bacteriol: 2004, 186(23);7936-43
[PubMed:15547265] [WorldCat.org] [DOI] (P p)

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)

Gregory S Allen, Katrin Steinhauer, Wolfgang Hillen, Jörg Stülke, Richard G Brennan
Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae.
J Mol Biol: 2003, 326(4);1203-17
[PubMed:12589763] [WorldCat.org] [DOI] (P p)

Katrin Steinhauer, Tanja Jepp, Wolfgang Hillen, Jörg Stülke
A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle.
Microbiology (Reading): 2002, 148(Pt 10);3277-3284
[PubMed:12368461] [WorldCat.org] [DOI] (P p)

Katrin Steinhauer, Gregory S Allen, Wolfgang Hillen, Jörg Stülke, Richard G Brennan
Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae.
Acta Crystallogr D Biol Crystallogr: 2002, 58(Pt 3);515-8
[PubMed:11856840] [WorldCat.org] [DOI] (P p)