RnpB

• Description: RNA component of RNase P
  
  

Gene name	rnpB
Synonyms	rnaP
Essential	yes PubMed
Product	RNA component of ribonuclease P (RNase P) (catalytic subunit, ribozyme)
Function	cleavage of precursor sequences from the 5' ends of pre-tRNAs
Interactions involving this RNA in SubtInteract: RnpB
Gene length	401 bp
Immediate neighbours	ypsC, ypsB
Gene sequence (+200bp)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

Rnases, translation, ncRNA, essential genes

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• essential PubMed

Database entries

• BsubCyc: BSU_misc_RNA_35

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:
  • 2A64 (from Geobacillus stearothermophilus)
  • 1NBS (specificity domain)
  • 3OKB (complex of RnpA with the RNA RnpB and tRNA, from Thermotoga maritima) PubMed

The RNA

• Biological activity: 5' end maturation of precursor tRNAs

• RNA family:

• cofactor: divalent cations (Ca(II) or Mg (II)) for the stabilization of the complex with pre-tRNA PubMed

• Interactions: RnpA-RnpB PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/RNA

• Carol Fierke, University of Michigan, Ann Arbor, USA homepage
• Roland Hartmann, Marburg University, Germany homepage