RelA

• Description: GTP pyrophosphokinase (stringent response)
  
  

Gene name	relA
Synonyms
Essential	no
Product	GTP pyrophosphokinase
Function	synthesis and degradation of (p)ppGpp, triggering the stringent response
Gene expression levels in SubtiExpress: relA
Metabolic function and regulation of this protein in SubtiPathways: relA
MW, pI	84 kDa, 6.964
Gene length, protein length	2202 bp, 734 aa
Immediate neighbours	dtd, apt
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU27600

Phenotypes of a mutant

• requirement for valine PubMed
• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
• mutation in relA results increased motility and chaining PubMed
• relA mutation increases flagellin production via elevated SigD level PubMed

Database entries

• BsubCyc: BSU27600

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)

• Protein family: relA/spoT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal ppGpp hydrolase domain PubMed
  • central ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27600

• Structure:

• UniProt: O54408

• KEGG entry: [3]

• E.C. number: 2.7.6.5

Expression and regulation

• Operon: relA-dtd PubMed

• Expression browser: relA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 244 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 573 PubMed

Biological materials

• Mutant: relA mutant PubMed - note that relA mutants are prone to suppressor mutations in the ywaC or yjbM loci PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Jue D Wang, University of Wisconsin–Madison [4]