PcrA

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Revision as of 13:45, 7 October 2014 by Fabian Commichau (talk | contribs) (Biological materials)
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  • Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

Gene name pcrA
Synonyms yerF
Essential yes PubMed
Product ATP-dependent DNA helicase
Function plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI 83 kDa, 5.767
Gene length, protein length 2217 bp, 739 aa
Immediate neighbours pcrB, ligA
Sequences Protein DNA DNA_with_flanks
Genetic context
PcrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PcrA expression.png















Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
    • dismantles RecA filaments PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s): YjcD

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus)
    • 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 114 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 250 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications