slrR

slrR
168

cofactor of SinR blocking elongation of SigD-dependent flagellar genes, antagonist of SlrA and SinR, has LexA-like autocleavage activity

Locus
BSU_34380
Molecular weight
17.43 kDa
Isoelectric point
9.63
Protein length
152 aaSequenceBlast
Gene length
459 bpSequenceBlast
Function
regulation of initiation of biofilm formation and of autolysis
Product
transcriptional regulator (Xre family), SlrA antagonist
Essential
no
Synonyms
slrR, yveJ, slr
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1396 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,530,101  3,530,559
Phenotypes of a mutant
smooth colonies on MsGG medium, no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
SlrR binds to and inhibits the activity of SlrA, SlrA indirectly stimulates the synthesis of SlrR by interacting with SinR
SlrR can bind to SinR and SinR directly represses the transcription of SlrR
SlrR indirectly derepresses its own gene. The heterocomplex of SlrR-SinR binds within the fliE and fliI open reading frames to prevent transcription elongation PubMed
repression of transcription of  ''lytA-lytB-lytC and lytF'' PubMed
autocleavage PubMed
Protein family
Xre family
Domains
HTH cro/C1-type domain (aa 6-61) (according to UniProt)
Sin domain (aa 113-151) (according to UniProt)
Structure
P71049 (AlphaFold)
Modification
subject to self-cleavage via a LexA-like autopeptidase, this involves ClpC and requires DegU PubMed
Effectors of protein activity
interaction with SinR triggers binding of SlrR to the promoters of ''lytA-lytB-lytC and lytF'', resulting in their repression PubMed
Paralogous protein(s)
SinR
Expression and Regulation
Operons
Genes
slrR-pnbA
Description
PubMed
Regulatory mechanism
Abh: activation, PubMed, in abh regulon
SinR: repression, PubMed, in sinR regulon
AbrB: repression, PubMed, in abrB regulon
Additional information
induction by sequestration of SinR by SinI, SlrA PubMed or by SlrR itself PubMed
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slrR->pnbA

2025-03-16 17:36:47

Jstuelk

119

3358c5e18cdab4c493893062172b97a17de9a83a

95EEFC91581CAC91E7EB53DE46FEEBE061AAC2B5

Biological materials
Mutant
MGNA-A089 (slr::erm), available at the NBRP B. subtilis, Japan
GP955 (slrR-''pnbA::cat''), available in Jörg Stülke's lab
BKE34380 (slrR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATATGAAATTCTCCTC,  downstream forward: _UP4_TGATGATCGGTTAAAGGGCT
BKK34380 (slrR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATATGAAATTCTCCTC,  downstream forward: _UP4_TGATGATCGGTTAAAGGGCT
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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920F91E748EE079FF864011D9052B073567C41E4

Page visits: 7949

Time of last update: 2025-04-03 09:48:49

Author of last update: Jstuelk