LysA

• Description: diaminopimelate decarboxylase
  
  

Gene name	lysA
Synonyms
Essential	no
Product	diaminopimelate decarboxylase
Function	biosynthesis of lysine
Gene expression levels in SubtiExpress: lysA
Metabolic function and regulation of this protein in SubtiPathways: lysA
MW, pI	48 kDa, 5.007
Gene length, protein length	1317 bp, 439 aa
Immediate neighbours	ypuA, spoVAF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU23380

Phenotypes of a mutant

• auxotrophic for lysine PubMed

Database entries

• BsubCyc: BSU23380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Meso-2,6-diaminoheptanedioate = L-lysine + CO₂ (according to Swiss-Prot)

• Protein family: Orn/Lys/Arg decarboxylase class-II family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23380

• Structure: 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed

• UniProt: P23630

• KEGG entry: [3]

• E.C. number: 4.1.1.20

Expression and regulation

• Operon: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA PubMed

• Expression browser: lysA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 627 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2286 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5990 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1660 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5360 PubMed

Biological materials

• Mutant:
  • 1A615 ( lysA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: