MurD

• Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
  
  

Gene name	murD
Synonyms
Essential	yes PubMed
Product	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murD
Metabolic function and regulation of this protein in SubtiPathways: murD
MW, pI	49 kDa, 5.566
Gene length, protein length	1353 bp, 451 aa
Immediate neighbours	mraY, spoVE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

The gene

Basic information

• Locus tag: BSU15200

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15200

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (according to Swiss-Prot)

• Protein family: murCDEF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15200

• Structure:

• UniProt: Q03522

• KEGG entry: [3]

• E.C. number: 6.3.2.9

Expression and regulation

• Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed

• Expression browser: murD PubMed

• Sigma factor:

• Regulation:
  • expressed during vegatative growth PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 235 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1679 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: