ThiF

• Description: hydroxyethylthiazole phosphate biosynthesis
  
  

Gene name	thiF
Synonyms	yjbU
Essential	no
Product	unknown
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiF
Metabolic function and regulation of this protein in SubtiPathways: thiF
MW, pI	36 kDa, 5.619
Gene length, protein length	1008 bp, 336 aa
Immediate neighbours	thiG, thiD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11700

Database entries

• BsubCyc: BSU11700

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): MoeB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU11700

• Structure: 1ZFN (from E. coli, 35% identity, 51% similarity) PubMed

• UniProt: O31619

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: thiF PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 323 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 790 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: