hemA

hemA
168

glutamyl-tRNA reductase

Locus
BSU_28170
Molecular weight
50.68 kDa
Isoelectric point
5.31
Protein length
455 aaSequenceBlast
Gene length
1368 bpSequenceBlast
Function
porphyrin biosynthesis
Product
glutamyl-tRNA reductase
Essential
no
E.C.
1.2.1.70
Synonyms
hemA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0373 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,877,766 → 2,879,133
The protein
Catalyzed reaction/ biological activity
(S)-4-amino-5-oxopentanoate + NADP+ + tRNAGlu --> H+ + L-glutamyl-tRNAGlu + NADPH (according to UniProt)
Protein family
Glutamyl-tRNA reductase family (single member, according to UniProt)
Structure
4N7R (PDB) (from Arabidopsis thaliana, 32% identity) PubMed
P16618 (AlphaFold)
Expression and Regulation
Operons
Genes
hemA-hemX-hemC-hemD-hemB-hemL
Description
PubMed
Regulation
induced by hydrogen peroxide (PerR) PubMed
Regulatory mechanism
PerR: repression, PubMed, in perR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hemA→hemL

2025-03-29 16:29:02

ghost

108

64840dd729762069005c4bd541ed0ddec6e74ace

168BC8B91CBFE3EA050DA8A9187C8C67D6BBE4F9

Biological materials
Mutant
BKE28170 (ΔhemA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGCTCTAATTACACCCC,  downstream forward: _UP4_CCACTTGTAAGTGAGTGAAA
BKK28170 (ΔhemA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGCTCTAATTACACCCC,  downstream forward: _UP4_CCACTTGTAAGTGAGTGAAA
References
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B1413EB525000744BE8E429E760220A13BC4530D

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Time of last update: 2025-04-06 13:44:23

Author of last update: Melvin.boenninger