Fni

• Description: isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	fni
Synonyms	ypgA, idi
Essential	no
Product	isopentenyl diphosphate isomerase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: fni
MW, pI	22 kDa, 6.101
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	ypzI, ypfD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

The gene

Basic information

• Locus tag: BSU22870

Database entries

• BsubCyc: BSU22870

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)

• Protein family: IPP isomerase type 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22870

• Structure:
  • 1P0N (complex with FMN)
  • 1P0K

• UniProt: P50740

• KEGG entry: [2]

• E.C. number: 5.3.3.2

Expression and regulation

• Operon:

• Expression browser: fni PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175] [WorldCat.org] [DOI] (P p)

Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687] [WorldCat.org] [DOI] (P p)