SpoVAD

• Description: essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2+) into developing spores, likely germination protein
  
  

Gene name	spoVAD
Synonyms
Essential	no
Product	binding protein for DPA
Function	likely germination protein
Gene expression levels in SubtiExpress: spoVAD
Metabolic function and regulation of this protein in SubtiPathways: spoVAD
MW, pI	35 kDa, 5.243
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	spoVAEB, spoVAC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU23410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • maybe involved in spore germination PubMed
  • binding of both DPA(2,6) and Ca(2+)-DPA(2,6) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer surface of the inner spore membrane PubMed

Database entries

• Structure: 3LM6

• UniProt: P40869

• KEGG entry: [3]

• E.C. number:

Additional information

• 6,500 molecules are present per spore PubMed

Expression and regulation

• Operon: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA PubMed

• Expression browser: spoVAD PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:
  • 6,500 molecules are present per spore PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA