NprE

• Description: extracellular neutral protease B
  
  

Gene name	nprE
Synonyms
Essential	no
Product	extracellular neutral protease B
Function	degradation of proteins
Gene expression levels in SubtiExpress: nprE
MW, pI	56 kDa, 7.481
Gene length, protein length	1563 bp, 521 aa
Immediate neighbours	yktD, ylaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids, proteolysis

This gene is a member of the following regulons

AbrB regulon, ScoC regulon

The gene

Basic information

• Locus tag: BSU14700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Similar, but not identical, to that of thermolysin (according to Swiss-Prot)

• Protein family: peptidase M4 family (according to Swiss-Prot)

• Paralogous protein(s): NprB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 1NPC (of Bacillus cereus)

• UniProt: P68736

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nprE (according to DBTBS)

• Expression browser: nprE PubMed

• Sigma factor:

• Regulation:
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)