PGP1389

pGP1389: click to enlarge

General comments

Integrative plasmid for the fusion of the C-terminus of a protein to a Strep tag, keeping the control of expression under control of the natural promotor of the target gene.

• E.coli: multicopy plasmid, select for ampicillin
• B. subtilis: no ori, select for spectinomycin

Hints for the utilisation of the plasmid

• amplify the last 600bp of your gene of interest,
• skip the Stopp-Codon of the gene in the reverse primer
• take care that the integration is in-frame

The reference

Martin Lehnik-Habrink, Joseph Newman, Fabian M Rothe, Alexandra S Solovyova, Cecilia Rodrigues, Christina Herzberg, Fabian M Commichau, Richard J Lewis, Jörg Stülke
RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli.
J Bacteriol: 2011, 193(19);5431-41
[PubMed:21803996] [WorldCat.org] [DOI] (I p)