PAC6

From SubtiWiki

Revision as of 13:53, 3 June 2013 by Jstuelk (talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to: navigation, search

pAC6: click to enlarge

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC5, pAC7

sequencing primer:

PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

Return to Plasmids

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PAC6&oldid=192290"