Fni

• Description: isopentenyl diphosphate isomerase
  
  

Gene name	fni
Synonyms	ypgA
Essential	no
Product	isopentenyl diphosphate isomerase
Function	lipid metabolism
Gene expression levels in SubtiExpress: fni
MW, pI	22 kDa, 6.101
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	ypzI, ypfD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, membrane proteins

The gene

Basic information

• Locus tag: BSU22870

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)

• Protein family: IPP isomerase type 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1P0N (complex with FMN), 1P0K

• UniProt: P50740

• KEGG entry: [2]

• E.C. number: 5.3.3.2

Expression and regulation

• Operon:

• Expression browser: fni PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Motoki Takagi, Kazuhide Kaneda, Tomohiro Shimizu, Yoichi Hayakawa, Haruo Seto, Tomohisa Kuzuyama
Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase.
Biosci Biotechnol Biochem: 2004, 68(1);132-7
[PubMed:14745175] [WorldCat.org] [DOI] (P p)

Stefan Steinbacher, Johannes Kaiser, Stefan Gerhardt, Wolfgang Eisenreich, Robert Huber, Adelbert Bacher, Felix Rohdich
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
J Mol Biol: 2003, 329(5);973-82
[PubMed:12798687] [WorldCat.org] [DOI] (P p)