MscL

• Description: large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment
  
  

Gene name	mscL
Synonyms	ywpC
Essential	no
Product	large conductance mechanosensitive channel protein
Function	resistance to osmotic downshockglycine betaine export
Gene expression levels in SubtiExpress: mscL
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	14 kDa, 4.649
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	ywpD, ywpB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU36360

Phenotypes of a mutant

sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mscL family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• Structure: 3HZQ (MscL of Staphylococcus aureus) PubMed

• UniProt: P94585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mscL PubMed

• Expression browser: mscL PubMed

• Sigma factor:

• Regulation: expressed in logarithmic phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A957 ( mscL::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

Erhard Bremer, University of Marburg, Germany homepage

References

Original Publications

Thijs R H M Kouwen, Erik N Trip, Emma L Denham, Mark J J B Sibbald, Jean-Yves F Dubois, Jan Maarten van Dijl
The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.
Antimicrob Agents Chemother: 2009, 53(11);4702-11
[PubMed:19738010] [WorldCat.org] [DOI] (I p)

Zhenfeng Liu, Chris S Gandhi, Douglas C Rees
Structure of a tetrameric MscL in an expanded intermediate state.
Nature: 2009, 461(7260);120-4
[PubMed:19701184] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Ann E Cowan, Barbara Setlow, Peter Setlow
Levels and localization of mechanosensitive channel proteins in Bacillus subtilis.
Arch Microbiol: 2009, 191(5);403-14
[PubMed:19252899] [WorldCat.org] [DOI] (I p)

Thijs R H M Kouwen, Haike Antelmann, René van der Ploeg, Emma L Denham, Michael Hecker, Jan Maarten van Dijl
MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a hypotonic environment.
Proteomics: 2009, 9(4);1033-43
[PubMed:19160392] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Clara Boiangiu, Susanne Moses, Erhard Bremer
Responses of Bacillus subtilis to hypotonic challenges: physiological contributions of mechanosensitive channels to cellular survival.
Appl Environ Microbiol: 2008, 74(8);2454-60
[PubMed:18310427] [WorldCat.org] [DOI] (I p)

Paul G Wahome, Peter Setlow
Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.
Arch Microbiol: 2008, 189(1);49-58
[PubMed:17665170] [WorldCat.org] [DOI] (P p)

Paul G Wahome, Peter Setlow
The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis.
Arch Microbiol: 2006, 186(5);377-83
[PubMed:16897034] [WorldCat.org] [DOI] (P p)

P C Moe, P Blount, C Kung
Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.
Mol Microbiol: 1998, 28(3);583-92
[PubMed:9632260] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)