GlnK

• Description: two-component sensor kinase, regulation of the glsA-glnT operon
  
  

Gene name	glnK
Synonyms	ycbA, yzgA
Essential	no
Product	two-component sensor kinase
Function	regulation of the glsA-glnT operon
Gene expression levels in SubtiExpress: glnK
Interactions involving this protein in SubtInteract: GlnK
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	43 kDa, 5.731
Gene length, protein length	1161 bp, 387 aa
Immediate neighbours	glsA, glnL
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, utilization of amino acids, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU02440

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of GlnL

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: four transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GlnK-GlnL

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P40758

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: glnK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)