DgcW

• Description: potential diguanylate cyclase and phosphodiesterase
  
  

Gene name	ykoW
Synonyms
Essential	no
Product	potential diguanylate cyclase and phosphodiesterase
Function	unknown
Gene expression levels in SubtiExpress: ykoW
MW, pI	84 kDa, 6.823
Gene length, protein length	2247 bp, 749 aa
Immediate neighbours	ykoV, ykoX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU13420

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a central GGDEF domain and a C-terminal EAL domain PubMed
  • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34311

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykoW PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP850 (ykoW::cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Additional publications: PubMed

Kazushi Suzuki, Paul Babitzke, Sidney R Kushner, Tony Romeo
Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
Genes Dev: 2006, 20(18);2605-17
[PubMed:16980588] [WorldCat.org] [DOI] (P p)

M Y Galperin, T A Gaidenko, A Y Mulkidjanian, M Nakano, C W Price
MHYT, a new integral membrane sensor domain.
FEMS Microbiol Lett: 2001, 205(1);17-23
[PubMed:11728710] [WorldCat.org] [DOI] (P p)