RapJ

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P
  
  

Gene name	rapJ
Synonyms	ycdE
Essential	no
Product	response regulator aspartate phosphatase
Function	control of the phosphorelay
Gene expression levels in SubtiExpress: rapJ
Interactions involving this protein in SubtInteract: RapJ
MW, pI	44 kDa, 4.902
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	cwlK, ycdF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphorelay, transcription factors and their control

The gene

Basic information

• Locus tag: BSU02820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • dephosphorylates Spo0F-P PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide PhrC, the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. PubMed
• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • the interaction with PhrC inactivates RapJ PubMed

• Interactions:
  • RapJ-Spo0F, dephosphorylation of Spo0F-P PubMed
  • RapJ-PhrC PubMed

• Localization:

Database entries

• Structure:
  • 4GYO (complex RapJ-PhrC) PubMed

• UniProt: O34327

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapJ (according to DBTBS)

• Expression browser: rapJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: