PGP1080

pGP1080: click to enlarge

General comments

• Integrative plasmid for the fusion of GFP tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor, allows express of the downstream gene by the P(spac) promoter

ATTENTION: This plasmid encodes the dimeric GFP that tends to produce artifacts!!!

• constructed in Jörg Stülke's lab (C. Diethmaier)

• E.coli: low copy plasmid, select for ampicillin
• B. subtilis: no ori, select for erythromycin/lincomycin

• based on pMUTIN-FLAG

Hints for the usage

• amplify the last 300bp of your gene of interest
• skip the stop codon of the gene in the reverse primer
• take care that the integration is in-frame
• do not use the XmaIII site for cloning, as GFP is inserted via this restriction site